Investigator:Jinbin Xu
Title:Quantitative autoradiography, genetics, and biochemistry studies
Date of Request:02/09/2021
Aim 1:Specific Aims: Specific Aim 1: To biochemically quantify the amount of amyloid-beta and tau species in specific regions of the frozen brain regions by mass spectrometry in DIAN-TU, DIAN-obs, and non-AD age-matched controls. Additional biochemical measures of other biomolecules will also be explored: alpha-synuclein, APP, ApoE, inflammatory markers, as well as various synaptic & neuronal markers.
Aim 2:Specific Aim 2: Compare the amount of soluble and insoluble amyloid-beta and tau species between active gantenerumab, active sola, placebo, and control cases including DIAN-obs and non-AD controls to determine the effects of drug treatment on amyloid-beta and tau species.
Aim 3:Specific Aim 3: To quantify by immunohistochemistry the amount of amyloid-beta and tau pathology and astrocytic, glial, alpha-synuclein, and TDP-43 in specific areas of the fixed brain regions in DIAN-TU, DIAN-obs, and non-AD age-matched controls. Additional immunohistochemistry measures of other biomolecules will also be explored, for example, APP, ApoE, inflammatory markers, as well as various synaptic & neuronal markers.