Search DIAN Tissue Requests

In order to avoid the situation where two investigators study the same research question, please search our database to determine if your topic has already been studied. If you find that your topic or a related topic has already been submitted, you may wish to contact the investigator to inquire about his/her findings to determine how you might proceed. You may wish to collaborate or modify your request to avoid overlap. The results below reflect requests made since online requests have been accepted. As such, not all fields will have data as certain information, such as aims, were not collected until recently. If an entry has been assigned an ID # (e.g. DIAN-T1004), the full request has been submitted and is either approved, disapproved or in process.

Displaying 1 - 10 of 108

Investigator:

P. Gleeson

Title:

Impact of familial Alzheimer’s disease mutations on APP trafficking and processing, and neuronal function

Date of Request:

06/23/2024

Status:

pending approval

ID:

DIAN-T2404

Aim 1:

To compare the trafficking pathways of wild type APP and familial Swedish APP mutant in human iPSC derived neurons

Aim 2:

To define the role of the Golgi in the processing of the familial Swedish APP mutant in the secretory pathway of iPSC-derived neurons

Aim 4:

To assess the impact of Golgi fragmentation, mediated by APP processing, on neuronal function

Investigator:

Randall Bateman

Title:

Accuracy and value of mid-throughput technology for biomarker measurement in the context of DIAN

Date of Request:

05/07/2024

ID:

DIAN-T2403

Aim 1:

Comparison of mid-throughput to one at a time measurements

Aim 2:

Comparison of mid-throughput to high-throughput measurements

Aim 3:

Non-inferiority assessment of mid-throughput performance to assess amyloid positivity

Investigator:

Kenneth Shepard

Title:

Hybrid CMOS/Brain-Organoid Reservoir Computing

Date of Request:

04/25/2024

Status:

approved

ID:

DIAN-T2402

Aim 1:

Aim 1. Integration of organoids onto wireless CMOS multielectrode arrays (MEAs). This task is about getting the hardware working for a prototype CMOS-organoid processor. This will rely heavily on the existing BISC hardware, a wireless 65k channel neural interface device, which communicates with an external wireless relay station. The BISC hardware allows organoids to be cultured directly on top of the interface chips, which should improve the quality of the recording and stimulating interface

Aim 2:

Aim 2. Demonstrate hybrid CMOS-organoid reservoir computing with the MNIST benchmark.

Investigator:

Josef Penninger

Title:

Generation of human AD lymphatic vessels

Date of Request:

01/09/2024

Status:

approved

ID:

DIAN-T2401

Aim 1:

Generation of human lymphatic vessel organoids using AD iPSC lines

Aim 2:

scRNA sequencing and profiling of lymphatic AD iPSC-derived organoids

Aim 3:

Functional characterization of lymphatic AD organoids under microfluidic draining systems

Investigator:

David Gate

Title:

Spatial transcriptomics on vaccinated AD brains

Date of Request:

09/18/2023

Status:

pending approval

ID:

DIAN-T2309

Aim 1:

Identify spatial transcriptomic changes associated with Aβ vaccination

Investigator:

Laurent Roybon

Title:

Models and therapies for Alzheimer's disease

Date of Request:

08/16/2023

Status:

pending approval

ID:

DIAN-T2308

Aim 1:

Generate humanized models of early and advanced AD cellular pathogenesis

Aim 2:

Use humanized models to explore a gene therapy for disease progression in AD

Investigator:

Sally Temple

Title:

Assessing neurovascular interactions due to ADRD mutations

Date of Request:

08/15/2023

Status:

pending approval

ID:

DIAN-T2307

Aim 1:

We will differentiate iPSCs with ADRD mutations versus controls into cerebral organoids and blood vessel cells. The goal is to examine the impact of the APP mutation on the organoids and blood vessel cells separately and interacting in a vascularized organoid.

Aim 2:

The vascularized organoids will be sectioned and stained for Abeta, p-tau and the staining in different cell types will be quantified.

Aim 3:

The conditioned medium produced by the vascularized organoids will be assessed for pathological markers and inflammatory factors.

Aim 4:

The vascularized organoids will be dissociated for single cell -omics studies.

Investigator:

Ji Yeun Hur

Title:

Characterization of gamma-secretase complexes in fibroblasts and iPSCs from PS1 or PS2 FAD mutations

Date of Request:

06/27/2023

Status:

pending approval

ID:

DIAN-T2306

Aim 1:

Characterize gamma-secretase complexes in fibroblasts and iPSCs

Aim 2:

Characterize the gamma-secretase activity in fibroblasts and iPSCs

Investigator:

Bart De Strooper

Title:

the role of presenilin mutation in microglia cell states

Date of Request:

04/14/2023

Status:

approved

ID:

DIAN-T2305

Aim 1:

Profiling the gamma-secretase substrate proteome in PSEN1 H163R iPSC-derived microglia.

Aim 2:

Characterizing the transcriptional and functional phenotype of human PSEN1 H163R microglia in a humanized chimeric AD mouse model.

Investigator:

Carlos Cruchaga

Title:

Functional characterization of brain circular RNAs in Alzheimer disease using induced pluripotent stem cell models

Date of Request:

04/04/2023

Status:

approved

ID:

DIAN-T2304

Aim 1:

To analyze the role of circHOMER1 in AD-related molecular phenotypes using cellular models