Search DIAN Tissue Requests
Sally Temple
Human iPSC model of Cerebro-Vascular Interactions in ADRD
07/09/2024
approved
DIAN-T2405
Generate and optimize a human cerebro-vascular model of PSEN1 and APP mutation
Validation of the PSEN1 and APP cerebro-vascular model, including measures of face, construct, and predictive validity
P. Gleeson
Impact of familial Alzheimer’s disease mutations on APP trafficking and processing, and neuronal function
06/23/2024
approved
DIAN-T2404
To compare the trafficking pathways of wild type APP and familial Swedish APP mutant in human iPSC derived neurons
To define the role of the Golgi in the processing of the familial Swedish APP mutant in the secretory pathway of iPSC-derived neurons
To assess the impact of Golgi fragmentation, mediated by APP processing, on neuronal function
Randall Bateman
Accuracy and value of mid-throughput technology for biomarker measurement in the context of DIAN
05/07/2024
approved
DIAN-T2403
Comparison of mid-throughput to one at a time measurements
Comparison of mid-throughput to high-throughput measurements
Non-inferiority assessment of mid-throughput performance to assess amyloid positivity
Kenneth Shepard
Hybrid CMOS/Brain-Organoid Reservoir Computing
04/25/2024
approved
DIAN-T2402
Aim 1. Integration of organoids onto wireless CMOS multielectrode arrays (MEAs). This task is about getting the hardware working for a prototype CMOS-organoid processor. This will rely heavily on the existing BISC hardware, a wireless 65k channel neural interface device, which communicates with an external wireless relay station. The BISC hardware allows organoids to be cultured directly on top of the interface chips, which should improve the quality of the recording and stimulating interface
Aim 2. Demonstrate hybrid CMOS-organoid reservoir computing with the MNIST benchmark.
Josef Penninger
Generation of human AD lymphatic vessels
01/09/2024
approved
DIAN-T2401
Generation of human lymphatic vessel organoids using AD iPSC lines
scRNA sequencing and profiling of lymphatic AD iPSC-derived organoids
Functional characterization of lymphatic AD organoids under microfluidic draining systems
David Gate
Spatial transcriptomics on vaccinated AD brains
09/18/2023
pending approval
DIAN-T2309
Identify spatial transcriptomic changes associated with Aβ vaccination
Laurent Roybon
Models and therapies for Alzheimer's disease
08/16/2023
approved
DIAN-T2308
Generate humanized models of early and advanced AD cellular pathogenesis
Use humanized models to explore a gene therapy for disease progression in AD
Sally Temple
Assessing neurovascular interactions due to ADRD mutations
08/15/2023
approved
DIAN-T2307
We will differentiate iPSCs with ADRD mutations versus controls into cerebral organoids and blood vessel cells. The goal is to examine the impact of the APP mutation on the organoids and blood vessel cells separately and interacting in a vascularized organoid.
The vascularized organoids will be sectioned and stained for Abeta, p-tau and the staining in different cell types will be quantified.
The conditioned medium produced by the vascularized organoids will be assessed for pathological markers and inflammatory factors.
The vascularized organoids will be dissociated for single cell -omics studies.
Ji Yeun Hur
Characterization of gamma-secretase complexes in fibroblasts and iPSCs from PS1 or PS2 FAD mutations
06/27/2023
approved
DIAN-T2306
Characterize gamma-secretase complexes in fibroblasts and iPSCs
Characterize the gamma-secretase activity in fibroblasts and iPSCs
Bart De Strooper
the role of presenilin mutation in microglia cell states
04/14/2023
approved
DIAN-T2305
Profiling the gamma-secretase substrate proteome in PSEN1 H163R iPSC-derived microglia.
Characterizing the transcriptional and functional phenotype of human PSEN1 H163R microglia in a humanized chimeric AD mouse model.