Search DIAN Tissue Requests
Kenneth Shepard
Hybrid CMOS/Brain-Organoid Reservoir Computing
04/25/2024
pending approval
DIAN-T2402
Aim 1. Integration of organoids onto wireless CMOS multielectrode arrays (MEAs). This task is about getting the hardware working for a prototype CMOS-organoid processor. This will rely heavily on the existing BISC hardware, a wireless 65k channel neural interface device, which communicates with an external wireless relay station. The BISC hardware allows organoids to be cultured directly on top of the interface chips, which should improve the quality of the recording and stimulating interface
Aim 2. Demonstrate hybrid CMOS-organoid reservoir computing with the MNIST benchmark.
Josef Penninger
Generation of human AD lymphatic vessels
01/09/2024
pending approval
DIAN-T2401
Generation of human lymphatic vessel organoids using AD iPSC lines
scRNA sequencing and profiling of lymphatic AD iPSC-derived organoids
Functional characterization of lymphatic AD organoids under microfluidic draining systems
David Gate
Spatial transcriptomics on vaccinated AD brains
09/18/2023
pending approval
DIAN-T2309
Identify spatial transcriptomic changes associated with Aβ vaccination
Laurent Roybon
Models and therapies for Alzheimer's disease
08/16/2023
pending approval
DIAN-T2308
Generate humanized models of early and advanced AD cellular pathogenesis
Use humanized models to explore a gene therapy for disease progression in AD
Sally Temple
Assessing neurovascular interactions due to ADRD mutations
08/15/2023
pending approval
DIAN-T2307
We will differentiate iPSCs with ADRD mutations versus controls into cerebral organoids and blood vessel cells. The goal is to examine the impact of the APP mutation on the organoids and blood vessel cells separately and interacting in a vascularized organoid.
The vascularized organoids will be sectioned and stained for Abeta, p-tau and the staining in different cell types will be quantified.
The conditioned medium produced by the vascularized organoids will be assessed for pathological markers and inflammatory factors.
The vascularized organoids will be dissociated for single cell -omics studies.
Ji Yeun Hur
Characterization of gamma-secretase complexes in fibroblasts and iPSCs from PS1 or PS2 FAD mutations
06/27/2023
pending approval
DIAN-T2306
Characterize gamma-secretase complexes in fibroblasts and iPSCs
Characterize the gamma-secretase activity in fibroblasts and iPSCs
Bart De Strooper
the role of presenilin mutation in microglia cell states
04/14/2023
approved
DIAN-T2305
Profiling the gamma-secretase substrate proteome in PSEN1 H163R iPSC-derived microglia.
Characterizing the transcriptional and functional phenotype of human PSEN1 H163R microglia in a humanized chimeric AD mouse model.
Carlos Cruchaga
Functional characterization of brain circular RNAs in Alzheimer disease using induced pluripotent stem cell models
04/04/2023
approved
DIAN-T2304
To analyze the role of circHOMER1 in AD-related molecular phenotypes using cellular models
Eain Murphy (David Butler, Co-Invesitigator)
Alzheimer’s Disease associated pathology induced by neurotropic viral infection.
03/03/2023
approved
DIAN- T2303
Establish biomarkers and activity of SU110 and SU134 in iPSC-derived models of familial AD.
Do herpesvirus infections of neurons and glial cells produce neurodegenerative changes similar to those seen in Alzheimer’s Disease?
Assess the efficacy of anti-tau intrabodies to lower AD pathology in familial iPSC derived neurons with APP V717I iPSC mutation
Cruchaga
Identification of mutation-specific networks: deep molecular profiling
02/13/2023
approved
DIAN-T2302
Identification and Characterization of Cell-Specific Transposable Elements Implicated on Alzheimer Disease and Healthy Aging
To generate neuron and microglia specific ATAC-seq, CAGE-seq, DNA methylation and long-reads RNA-seq