Search DIAN Tissue Requests

In order to avoid the situation where two investigators study the same research question, please search our database to determine if your topic has already been studied. If you find that your topic or a related topic has already been submitted, you may wish to contact the investigator to inquire about his/her findings to determine how you might proceed. You may wish to collaborate or modify your request to avoid overlap. The results below reflect requests made since online requests have been accepted. As such, not all fields will have data as certain information, such as aims, were not collected until recently. If an entry has been assigned an ID # (e.g. DIAN-T1004), the full request has been submitted and is either approved, disapproved or in process.

Displaying 81 - 90 of 112

Investigator:

Johannes Levin

Title:

Molecular detection of α-synuclein co-pathology in ADAD mutation carriers

Date of Request:

04/29/2021

Status:

approved

ID:

DIAN-T2104

Aim 1:

Use real time quaking induced conversion (RT-QuIC) for ante mortem detection of Synuclein pathology in ADAD mutation carriers

Aim 2:

Compare the data from RT-QuIC regarding in vivo detection of Synuclein pathology with data from post mortem examinations (manuscript in preparation).

Aim 3:

Within the time frame of 15 years EYO we will analyse the time-point of emergence of LB pathology as measured by RT-QuIC .

Aim 4:

We will analyse clinical and imaging data longitudinally comparing RT-QuIC positive and RT-QuIC negative participants.

Investigator:

Mariah S. Hahn

Title:

Targeting Dysregulated Synapse and Proteostasis Mechanisms in Alzheimer's Disease

Date of Request:

06/03/2021

Status:

approved

ID:

DIAN-T2105

Aim 1:

Develop a 3D culture system compatible with long-term (>3 month) iNeuron culture, extension, synapse formation and elimination, and quantitative assessment of neural circuit activity using “healthy” networks.

Aim 2:

Compare AD_(PSEN1)-iNeuron cultures to iNeuron cultures derived from unaffected family member controls with respect to Aβ, tau phosphorylation, synapse maintenance and proteostasis.

Investigator:

Randall J Bateman

Title:

Comprehensive analyses of the soluble microtubule binding region (MTBR)in Alzheimer disease progression.

Date of Request:

09/02/2021

Status:

approved

ID:

DIAN-T2106

Aim 1:

The Primary Aim of this study isto identify which tau species are novel and different inthe novelUCB vs. other antibodies which have been used to characterize the soluble tau in CSF of DIAN(e.g. Tau1 or E2814).

Aim 2:

The stage of the disease where additional MTBRisoforms begin to differ between MC and NC based on EYO and Aβ status andcompare this to the recently identified MTBR and p-tau mass spec measures of MTBR tau and p-tau.

Aim 3:

The longitudinal change (quantitative change / mean and SD) of thenovel MTBRfragmentsas it relates to disease stage and how this compares to the recently identified MTBR and p-tau mass specmeasures of MTBR tau and p-tau.

Aim 4:

The association between regional NFT tau burden (post-mortem) and MTBR (based on the novel antibody and Eisai antibody) to assess for the correlation between soluble and insoluble fractions of MTBR with AD related NFTs. Design:

Investigator:

Ronald Davis

Title:

Capturing the mitochondrial complexity in familial Alzheimer's disease

Date of Request:

09/15/2021

Status:

pending

ID:

DIAN-T2107

Aim 1:

Use high-content and high-throughput assays to quantify the MT dynamics phenotypes that develop in neurons derived from iPSCs of fAD subjects compared to age-matched and/or isogenic controls.

Aim 2:

Define the functional impairment in these neurons (IMM-inner mitochondrial membrane potential).

Aim 3:

Interrogate the function of the electron transport chain (ETC) in these neurons.

Aim 4:

Measure the structural integrity of MT, the ETC, and MT-related processes in these neurons by quantitative Western blotting of proteins

Investigator:

Dan Nicholson

Title:

High-resolution multiplex localization of Alzheimer’s disease risk and resilience factors

Date of Request:

09/23/2021

Aim 1:

Determine the expression levels, localization, and co-localization patterns of proteins in the m109 and BIN1 protein clusters in relation to synaptic markers. Synaptic changes have long been recognized to be tightly linked to AD severity (9). We will determine the expression patterns of the two protein clusters relative to excitatory and inhibitory synapses using antibodies specific for excitatory synapses (e.g., AMPA- and NMDA-type receptors and PSD-95), inhibitory synapses (e.g., vesicular GABA transporter/VGAT and gephyrin), and presynaptic terminals (e.g., synapsin).

Aim 2:

Determine the expression levels, localization, and co-localization patterns of proteins in the m109 and BIN1 protein clusters in relation to AD pathology. Probable AD is confirmed at autopsy by the presence of amyloid plaques and tangles (10). We will determine the expression patterns of the two protein clusters as a function of proximity to regions with amyloid plaques (using a 6E10 antibody), neurofibrillary tangles (using a tau13 antibody), astrocytes (using a GFAP antibody), and microglia (using an Iba1 antibody).

Investigator:

Cruchaga

Title:

Multi-Omics CSF and plasma signature of ADAD

Date of Request:

10/16/2021

Aim 1:

To identify ADAD-specific multi-omic (proteomics and metabolomic) signatures CSF and plasma

Aim 2:

To identify multi-omic (metabolomics and proteomics) plasma signatures and QTLs in ADAD

Investigator:

Jee Hoon Roh/Jae-Hong Lee

Title:

Epigenetic analyses to assess the resilience among DIAN mutation carriers

Date of Request:

12/15/2021

Aim 1:

To investigate the potential epigenetic causes of resilience among DIAN mutation carriers who are discordant in disease courses measured by biomarkers.

Investigator:

Giuseppina Tesco

Title:

Study of endolysosomal alterations (including exosomes) in brain cells cultures derived from fDA iPSC lines and isogenic controls

Date of Request:

01/25/2022

Status:

approved

ID:

DIAN-T2201

Aim 1:

Determine the effect of mutant APP on exosome dynamics in CNS cells in human AD models.

Investigator:

Antonio Boza Serrano

Title:

Galectin-3 as a prognostic biomarker to monitor Autosomal dominant Alzheimer's Disease's progression

Date of Request:

04/27/2022

Status:

not approved

ID:

DIAN-T2202

Aim 1:

We aim to evaluate if gal3 levels are elevated in ADAD and if they might track the progression of AD pathology.

Aim 2:

We want to compare the levels of gal3 with the levels of the classic biomarkers (amyloid-beta 42, total tau, p-tau (181, 217, etc), neurofilaments, precuneus SURV and PIB-Pet) as well as with global cognitive test (MMSE) and clinical measures (CDR total and SB) scores from the DIAN-obs

Investigator:

Patrick Oeckl

Title:

Investigation of plasma Beta-Synuclein levels in asymptomatic and symptomatic autosomal dominant Alzheimer´s disease

Date of Request:

06/20/2022

Status:

approved

ID:

DIAN-T2204

Aim 1:

To investigate if blood levels of the synaptic marker Beta-Synuclein are already elevated in asymptomatic ADAD

Aim 2:

To estimate a time course of blood Beta-Synuclein changes in ADAD based on EYO and comparison with other blood/CSF biomarkers

Aim 3:

Relationship of blood Beta-Synuclein levels in ADAD with other fluid and imaging biomarkers, brain atrophy and cognitive impairment