Search DIAN Tissue Requests
Young-Pearse
Investigating Cell-type dependent phenotypes in familial Alzheimer's disease
12/27/2016
DIAN-T1701
To determine if there are differential effects of neuronal subtype on APP processing with fAD mutation
To determine if there are differential effects of neuronal subtype on tau proteostasis with fAD mutation
Jacques P. Tremblay
Development of a treatment of Alzheimer based on the editing of the Amyloid Precuror Protein gene with the CRISPR system
10/24/2016
DIAN-T1613
To correct the APP gene in 293T cells and in Alzheimer cells with a pair of gRNA (targeting sequences in intron 15 or 16) and with dCas9-FokI
MPIs: Tom Montine and Mike MacCoss
Molecular Phenotyping in Alzheimer's Disease
10/21/2016
DIAN-T1612
We will collect proteomics data on post-mortem brain samples using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) strategy called data independent acquisition.
Leverage high-dimensional proteomics data to identify and understand the molecular signatures of three groups that will enable precision medicine for AD: (i) different genetic risk, (ii) common co-morbidities, and (iii) resilience to AD neuropathologic change.
We will make our data available through a novel cloud based solution, called the Chorus Project (http://chorusproject.org), engineered to enable big data reanalysis by the community of scientists.
Hideyuki Okano
In-depth characterization of iPSC-derived neuronal models carrying familial dementia mutations
9/30/2016
DIAN-T1611
Generation and development of human neuronal models with dementia-related dysfunctions
To explore molecular mechanism of neuronal malfunctions by FAD-linked mutations
Dr. A Claudio Cuello
Investigating early NGF dysmetabolism as a source of novel biomarkers in ?silent? stages of Alzheimer?s disease
9/23/2016
DIAN-T1610
To investigate plasma markers of NGF dysmetabolism as a source of biomarkers in pre-clinical AD
To investigate CSF markers of NGF dysmetabolism as a source of biomarkers in pre-clinical AD
Alice PEBAY
Human iPSC-derived organoids to study Alzheimer?s disease
9/9/2016
DIAN-T1609
to generate cortical organoids from iPSCs of AD patients and controls
to generate iPSCs from APP mutation fibroblasts
To study pathological events of AD in organoids
Sidney Strickland
Aβ-specific fibrin fragment resistant to fibrinolysis in the CSF and plasma of familial AD patients with HCHWA
9/7/2016
DIAN-T1608
Analyze the level of fibrin degradation products in the antemortem CSF of HCHWA patients and compare levels to sporadic AD cases and non-demented controls.
Analyze the level of an A?-specific fibrin fragment resistant to fibrinolysis in the plasma of AD patients with HCHWA and compare levels to sporadic AD cases and non-demented controls.
Randall Bateman
DIAN-ADNI Comparison study
7/19/2016
DIAN-T1607
characterize the stages of preclinical AD with amyloid PET and cerebrospinal fluid (CSF) concentrations of Aβ and tau and phosphorylated tau (p-tau) and determine whether both groups demonstrate initial cerebral amyloidosis that is followed by the development of neurodegeneration prior to onset of A
In both ADAD and LOAD, determine whether initial symptoms of AD are characterized by subjective reports (self-reported and those of a study partner) of the gradual onset of memory impairment as well as by deficits in objective measures of episodic memory
In both LOAD and ADAD, determine whether there is a pattern of disease progression that is marked by intra-individual global cognitive and functional decline that culminates in death.
Characterize other similarities and any differences in AD phenotypes between LOAD and ADAD
Mathias Jucker
Neurofilament light chain in blood serum as marker of disease progression in neurodegenerative diseases
6/29/2016
DIAN-T1606
Determine whether and how many years before the estimated time point of symptom onset (EYO) mutation carriers (MC) of autosomal dominant Alzheimer?s disease (ADAD) show altered blood (serum) levels of neurofilament light chain (NfL) compared to non-mutation carriers (nMC).
Test whether serum levels of NfL are correlated with previous measured CSF levels and are associated with longitudinal changes in PIB-PET, FDG-PET, and structural MRI, resting state fMRI, with other CSF biomarkers, clinical symptoms and performance
Perminder S Sachdev
Identification and preliminary validation of plasma protein, lipid and metabolite biomarkers in autosomal dominant Alzheimer?s disease
6/2/2016
DIAN-T1605
Identification of plasma protein biomarker candidates using two discovery proteomics approaches (iTRAQ and SWATH-MS)
Targeted proteomics for validation of protein biomarker candidates and comparison of proteins changes in ADAD and LOAD
Identification of metabolite biomarkers using targeted and non-targeted metabolomics
Investigate and validate lipid and metabolite alterations using liquid chromatography coupled with mass spectrometry (LC-MS) in autosomal dominant Alzheimers disease